Chromusome segregation during mitosis explained


For the purpose of establishing a functional kinetocore, a microsaval-compulsory Ndc80 complex (Ndc80C) should be composed in the Kinetocore. The previous model believes that CENP-C is the main observer of the Ndc80C, this study showed that CENP-T is actually the main player in the Ndc80C recording of kinetocore. Credits: Osaka University

When the cell divides the process – the process is known as mitosis – its chromosome should be distributed and equally distributed in the cells of newly formed daughters. Although it is known to be extremely complicated and functioning of cellular components, its details remain unclear, which prevented efforts to develop treatment when mitochondrial.


A new study reported in the journal Biology of nature cell Introducing more profound knowledge in this process by revealing the details of how the protein complexes are in the centers of chromosomes, the centers are known. In these areas, the protein complex acts as anchors, through which cell structural organizers can distribute chromosomes within the cell.

This protein complex centromres known as kinetochores, which are long, thin, cylindrical structures called microtubules. In cells cell, microtubules can physically manipulate chromosomes, half of each chromosome in one cell's cell and another in the other.

In this new study, the University of Osaka emphasized the different components that make up the kinetokos. One of these groups is CCAN proteins, which are located in the entire cycle of the center and will act as a compulsory place for other micro-urolithic-association proteins when the cell division becomes. This work shows that CCAN's proteins subdivided the CENP-T transmitter to dominate the successful cell division by a protein complex called the Ndc80 complex that allows microbials to be attached to the chromosomes.

During mitosis, spindle microtubules in the hands of kinetochore formed centromere. After mitosis, chromosomes are divided into cells of the daughters. Credits: Osaka University

"We have analyzed whether a CENP-T road or a CENP-C road has been investigated for mitosis, which reduces the parts of these proteins that satisfy the Ndc80 complex," said Tatsua Fukava. "CENP-T mutants with domains do not obey Ndc80, but not similar to CENP-C mutants, have been identified as the failure of the chromosomes to segregate, avoid cell division and ultimately leading cell death."

The CNSP-T's essential role has been demonstrated by the introduction of the cell division through the Ndc80 complex, the team has also built chimeric constructions that are partially of CENP-T and CENP-C. It was confirmed that CENP-T and molecules associated with it are very important for mitosis. They also found that phosphorolation plays an important role in the compulsory obligatory regulatory process between the Ndc80 complex and CENP-T, and will gain additional evidence for measuring the dividing force produced by the micro-tube in the spindle.

Besides the gene pool: chromosome segregation explains the mitochondria

In case of lack of protein phosphorylation, the Ndc80C predominantly connects a CENP-C complex. After the protein phospholipation occurs, Ndc80C mainly connects CENP-T. Credits: Osaka University

"Our work clearly determines the previously consensus because it is a CENP-T, CENP-C that operates through the Ndc80 complex to provide accurate and timely chromosomal segregation through successful cell division," says lead author Márata Hara. "Conclusions can lead to therapeutic options for treatment of diseases involving dysfunction kinetochores and mitotic progression, including cancer."


Learn further:
The two-stage process is in the process of keeping key proteins in the cell section

Მet Information:
Masatoshi Hara et al, many phosphorylations control the hiring KMN network onto kinetochores, Biology of nature cell (2018). Doyle: 10.1038 / s41556-018-0230-0

Journal Reference:
Biology of nature cell

Provided by:
Osaka University