When the cell divides the process – the process is known as mitosis – its chromosome should be distributed and equally distributed in the cells of newly formed daughters. Although it is known to be extremely complicated and functioning of cellular components, its details remain unclear, which prevented efforts to develop treatment when mitochondrial.
A new study reported in the journal Biology of nature cell Introducing more profound knowledge in this process by revealing the details of how the protein complexes are in the centers of chromosomes, the centers are known. In these areas, the protein complex acts as anchors, through which cell structural organizers can distribute chromosomes within the cell.
This protein complex centromres known as kinetochores, which are long, thin, cylindrical structures called microtubules. In cells cell, microtubules can physically manipulate chromosomes, half of each chromosome in one cell's cell and another in the other.
In this new study, the University of Osaka emphasized the different components that make up the kinetokos. One of these groups is CCAN proteins, which are located in the entire cycle of the center and will act as a compulsory place for other micro-urolithic-association proteins when the cell division becomes. This work shows that CCAN's proteins subdivided the CENP-T transmitter to dominate the successful cell division by a protein complex called the Ndc80 complex that allows microbials to be attached to the chromosomes.
"We have analyzed whether a CENP-T road or a CENP-C road has been investigated for mitosis, which reduces the parts of these proteins that satisfy the Ndc80 complex," said Tatsua Fukava. "CENP-T mutants with domains do not obey Ndc80, but not similar to CENP-C mutants, have been identified as the failure of the chromosomes to segregate, avoid cell division and ultimately leading cell death."
The CNSP-T's essential role has been demonstrated by the introduction of the cell division through the Ndc80 complex, the team has also built chimeric constructions that are partially of CENP-T and CENP-C. It was confirmed that CENP-T and molecules associated with it are very important for mitosis. They also found that phosphorolation plays an important role in the compulsory obligatory regulatory process between the Ndc80 complex and CENP-T, and will gain additional evidence for measuring the dividing force produced by the micro-tube in the spindle.
"Our work clearly determines the previously consensus because it is a CENP-T, CENP-C that operates through the Ndc80 complex to provide accurate and timely chromosomal segregation through successful cell division," says lead author Márata Hara. "Conclusions can lead to therapeutic options for treatment of diseases involving dysfunction kinetochores and mitotic progression, including cancer."
The two-stage process is in the process of keeping key proteins in the cell section
Masatoshi Hara et al, many phosphorylations control the hiring KMN network onto kinetochores, Biology of nature cell (2018). Doyle: 10.1038 / s41556-018-0230-0