Cytotic NQO1 enzyme-activated near-infrared fluorescence imaging and photodynamic therapy with polymeric vesicles

Abstract image

The use of enzymes as a stimulating module can induce responsive polymeric nanostructures in site-specific selectivity. Since endogenous NAD (P) H: quinone oxidoreductase isozyme 1 (NQO1) is expressed in many types of tumors, we report information on the falsification of photosensitizer-conjugated polymeric vesicles, suggesting a reversal of the synergistic NQO1-derived signals. As a near-infrared (NIR) fluorescence emission and module of photodynamic therapy (PDT). For vesicles made from amphiphilic block copolymers containing quinone trimethyl-bound self-propelled side bonds and quinone-bridged photon sensors (coumarin and nile blue) embedded in a hydrophobic block, both fluorescence emission and PD are "initially" fluorescent. Due to the "double quenching" effects, ie quenching due to the accumulation of dyes and quenching from quinone PET (photosynthetic electron transfer). After integration with NQO1 positive vesicles, the cytosolic NQO1 enzyme induces self-deletion of quinone bonds and fluorogenic release of conjugated photosensitizers, which results in the involvement of NIR fluorescence emission and activated PDT. This process is accompanied by the conversion of vesicles to cross-micelles by hydrophilic nuclei and small-scale decomposition induced by dual drug release, which can be directly monitored for enhanced magnetic resonance (MR) imaging for vegetarians conjugated with DOTA (G). Hydrophobic bilayer. We further show that the above strategy can be successfully applied to NIR fluorescence imaging and tissue-specific PDT, both cellular and in vivo conditions.