Finding fast and cheap ways to detect specific strains of bacteria and viruses is critical to food safety, water quality, environmental protection and human health. However, the existing methods of detecting the strains of bacterial disease, such as E. coli Requires intensive biological cells cultures or DNA strengthening approaches, depending on expensive laboratory equipment.
Now, Washington University, University of California, electronic and computer equipment, Associate Professor of Economics and Technology University, Ankara, Turkey, and Josh hihati Economics and Technology University professor of molecular electronic device is adapted to that of the molecule break Junction to detect RNA from strain it E. coli It is known for the cause of the disease. The survey was published today (November 5) in the journal Nature of Nanotechnology.
"Reliable, effective and cheap identification and identification of specific strains of microorganisms E. coli Biology and Health Science is a big challenge, "said Hahatma -" Our technique can deteriorate rapid, direct detection pathogens, antimicrobial resistant bacterial strains and biomarkers for cancer. "
Hahahat and his team emphasized E. coli This is a common pathogen that can easily be found in the supply of food, but may not cause illness in a benign form. The worst tension E. coli, Entitled E. coli O157: H7 produces a toxic substance known as internal toxin which causes bloody diarrhea, kidney failure and death.
Single molecular break knot equipment consists of two metal electrodes with an atomic sharp interface that involves contact with liquid solutions such as RNA sequences from E.coli. As the electrodes are in contact and are removed, electrical compensation is used and the measure is measured. This process is repeated hundreds or thousands of times to determine a single molecule.
"We asked one of the questions about how consistent change is necessary to cause significant change of power transmission?" Hahahat said. "The smallest thing we can change is one base, so we decided to change the uniform base."
Testing of the RNA short-term dates of DNA with chemical installation E. coli The sequence represented by the toxin. Their conclusions have shown that the change in the electrical resistance of the RNA can be determined on the uniform basis, which will enable them not only to present E.coli, but also the specific strain of E.coli, which produces internal toxins.
"A system that can be selectively defined by DNA or RNA short sequences, provides a wide range of new ways of developing the electronic sensors platform," he adds. "Finally, we want to see the point where we can extract RNA samples of real organisms and measure their holding on the felt platform."
This work was supported by the National Science Foundation.
Materials provided University of California – Davis. Note: Edit content for style and length.