One of the front lines of DNA, which creates nucleobases cytosine and guanine, can be imprinted in polymer – shown by chemists in Warsaw, Denton and Milan. The resulting artificial negative, long-lasting length is functioning chemically as a normal string of deoxyribonucleic acid. In the end, it is possible to create DNA polymer implants that functionally correspond to the DNA fragments that constitute all four nuclei.
A year and a half ago, the group of Polish-American-Italian researchers created the DNA negative molecular implant. The molecular cavities, which are carefully designed polymer, are a real DNA chaos similar to chemically used for implantation. The first oligomer was "contaminated" with the polymer, which consisted of only six aden and thin nuclei forming the TATAAA sequence. Currently in Warsaw, the Institute of Physics of the Polish Academy of Physics (Professor Vladimir Kutner) is headed by North Texas University in Denton (USA) and Milan (Italy). The next step was taken. In the journal ACS Applied Materials and InterfacesResearchers have demonstrated the process of building a negative fragment of one of the DNA's directions, which contains other nuclei: cytosine and guanine.
"The oligonucleotate, which is confused with the polymer, is more than just described in our previous publication, but it was not a violation of the records, and the most important thing is that molecular implanting method can be used to stabilize negative strains of oligonucleulotides all the nuclei of decoxhibibilankalic acids," says Professor Kutner.
Each DNA molecule is a ribbon that is two long and constantly connected to the fiber. Each particle consists of multiple repetitions of nucleotides, each of which contains one nucleobase: Adenia (A), Guanine (G), Cytosine (C) or thymine (T). Since one of the parts of Atenin is included on the second of the thymine and Guinean cytosine, based on one of the DNA's layers, it is easy to reconstruct its additional partner. This mechanism not only increases the genetic code record, but also allows the DNA to transition into RNA transcription process, which is the first phase of protein synthesis.
"The DNA molecules are too long if they are corrected, measuring the length of centimeters, in normal conditions, the DNA has been turned into double and the polymer's complex structure is not only impossible but because there are different molecules of the same DNA So, as a rule, double-strand DNA testing, and so on c cut into fragments, which contains dozens of nucleotides, then it is possible to try to imprint in the polymer fragments of this length, "explains Dr. Agnieszka Pietrzyk-Le (IPC PAS).
In order to reduce polymer molecules, they are proposed in the solution of monomers or "building blocks" from which future future polymer formation is formed. Some monomers are selected to make self-assemble themselves around the molecules being imprinted. The mixture is then polymerized electrolymically. This electroplomerization causes the polymer's thin, fortified films from which the molecules are removed. Thus, polymer molecules are obtained by molecules, not only of size and shape, but also with local chemical properties.
"In our recent research we have shown that GCGGCGGC oligonucleotide, or one of the eight nuclear substances containing genetically important polymer, which contains genetically important role, and its presence increases the likelihood of nervousness," explained Dr. Katarina Bartol (IPC PAS).
The first polymeric negative, which was the rare aesthetic oligomer, was completely selective, which only used TATAAA molecules used to prepare polymer used in molecular clouds. Currently synthesized polymer, Guanine-Cytosine cavity is also selective, but this selection should still be desired. If the olegonucleotide reception differs from the GCGGCGGC oligonucleotide, which is used to notice that the difference is not noticeable. The researchers make this behavior between the strongest of gonny and cytosine, between Adenine and Tamin.
"DNA disadvantages are considered more DNA than DNA's natural DNA genuine DNA system that has a nucleotide core that is electronically contaminated, which molecules interfere with each other, such as introduction of positive sodium ions , Our molecular clouds are already electronically neutral, Mr. polymeric DNA using the analogy, the elimination of the research stage: neutralization ", – said Dr. Le-pietsik.
In the nearest future, researchers are going to develop developed techniques, more dense fragments of DNA, with oligonucleotides comprising at least a dozen or nucleotides. Polymer films produce such genetically important DNA fragments effectively with such molecular hawks. This will be possible after the polymer mass, which is filled with oligomose tests, becomes a polymer electrical conductor, and changes in these parameters can be easily detected. Another application will be available in the future. Polymer films filled in these fragments will be able to study new drugs focused on genetics, with fragments of dNA fragments and molecular cavities.