Surface chemistry of nanoparticles is a fundamental engineering parameter that regulates tumor targeting. The electrostatic assembly generates controlled polyelectrolyte complexes through the adsorption process and recharging the charge through compensation, using synthetic polynomials and natural biomacromolecules; It can shoot movies with distinctive hydration, load and presentation of functional groups. Here, we used an electrostatic layer assembly (LbL) to view 10 different surface chemistries for their suitability for human ovarian cancer benefits. in vitro. This screen has been detected on our screen-L-Aspartate, Pol-L-In combination with glutamate and hyaluronate, LbL nanoparticles have specificity for ovarian cancer, whereas sulfated poly (β-cyclodextrin) nanoparticles are targeted to abnormal stromal cells. We have confirmed advanced candidates for tumor homogeneity in the musculoskeletal model of metastatic disease and in patients with spherical ovarian cancer. The surface chemistry of nanoparticles has also been implicated in cell trafficking, indicating targeting strategies for cell membranes, caves, and perinuclear vesicles. Our results confirmed that LbL is a powerful tool for systematically engineering nanoparticles and for achieving a specific target.