Carnegie Mellon University researchers developed a synthetic molecule that can recognize and satisfy bilateral DNA or RNA normal physiological conditions. The molecule can create a new platform for the development of genetic conditions diagnostic and treatment methods. Their results were published Communications Chemistry.
The works were conducted by an international team of experts, including Carnegie Mellon's chemistry professor Daniele Lee, expert in peptide nucleic acid design, chemistry postdina shaige tadke and chemistry graduate Dineta Perrara, chemistry professor, and nuclear magnetic resonator expert Roberto Gil, and Arnab Mukherjee, Indian Institute of Computer Science Science tlebisa research and Pune.
"Since DNA's double-consuming structure was initially revealed by Watson and Crick, scientists have tried to form molecules that are in line with DNA and control the flow of genetic information." This is the first bipolar molecule that can cause dual stranded DNA or RNA biologically in appropriate conditions. "
DNA containing genetic information of all organisms consists of two nucleotides. Nucleotides connect hydrogen bonds to each other, creating a Watson-Crick base jackal network. While this base pair provides a relatively simple code for our genetic information, getting into a double helix to adjust the code is difficult due to the strong bonds between the base pairs.
Lee and his colleagues at the Institute of Carnegie Mellon University and the Center for Science and Technology at the Nucleic Acid Science and Technology Center (CNAST) are the barmolecular design and detection of design and development of gamma-peptide nucleic acids (gamma PNAs). The synthetic analogs of DNA and RNA, gamma PNA can be linked to a programmed genetic material (DNA or RNA) that causes a disease that allows them to find harmful sequences and the gene linking them to the genofoning.
The group created the double-faced gamma PNAs Janus Gamma PNAs. Juan PNA, named after the Roman god, can recognize and connect with both the DNA or RNA molecules.
The concept of bisexual recognition, which is the foundation of the Yunus Gamma PNA, was the first Nobel Prize laureate two decades ago, known for the field of salarolekular chemistry and other researchers.
The progress of this research was carried out with two obstacles. First of all, researchers were able to make only a small number of Gene bases, and these bases were significantly different in shape and size. These restrictions indicate that different genuine databases could only be reciprocated by the same base pairs and can not be used to identify more complex sequences of DNA and RNA like building blocks.
Secondly, it was hard to synchronize Janus bases with a canonical base pair. Comparative nature of the two sides of the junction bases molecules form hybridization and connect with each other in order to avoid DNA and RNA.
In the current study, Lee and colleagues will overcome these obstacles. They have created a complete set of new nuclear nucleic acid recognizable elements, the total number of which is greater than 16, which had all possible combinations of nuclei for genetic code. The use of YANUSA GAMMA PNA can be used for any combination of basal waters and mixed and compatible with the study and connection of complex genetic sequences.
Thadke decided to solve the chemical synthesis problem by issuing a new solution and a solid phase synthetic method to develop Janus Gamma PNAs. He also placed a mitigation of the prerequisite, which included in the PNA system, in order to prevent the filling of the self-government by hybridizing each of the Janus bases.
This new Yanus Gamma PNA has an extraordinarily highly compulsive energy and is the first to conquer the DNA or RNA dual Helix filled with canonical bases during physiologically appropriate ion power and temperature.
They do this advantage when dual stranded DNA and RNA molecules are "breathing" and bonds between the base pair of open fractions second. When this happens, the strings separated between the Janus PNA tab. If the base pairs do not match, the JUNU's PNA opens with the DNA molecule. But if they match the match, Jonas PNA connects both the molecules of the molecule.
Janus Gamma PNA has a wide range of biological and biomedical use. They can be created to regulate Genome DNA genome editing and transcription. They can also be worked out in a sequence – specifically and randomly to the RNA's secondary and tertiary structures that can not afford traditional anti-sexual agents and small-molecular ligons. For example, Yanus gamma PNAs may be programmed to re-enlarge RNA, which may lead to new treatment for neuromuscular and neurodegenerative disorders, including myantin dystrophy type 1 and hanthington disease, or non-congenital reenos, including pathogenic ribosomal and tlammerase RNA, genetic and Against infectious diseases to combat crime.
Technology starts by the startups as well as pharmaceutical companies for therapeutic development.
Image: Janus from far
Shivaji A. Thadke et al, the shape of selective bifacial recognition of double helical DNA, Communications Chemistry (2018). DOI: 10.1038 / s42004-018-0080-5