The development of neurons is often regulated by the degree of molecules of the molecules that can attract the neurological migration or neuronal prediction or profit when presented in concentration gradients or chemics. However, many details about the process are mostly unused.
The Research Group of the University of Hong Kong (CityU) has solved this problem through the establishment of a new device in the correct and systematic manner and published their findings in the study article in the journal Nature Communications, Called "high throughput three-dimensional chemotactic assays revealing steepness on the complexity of the neuron sensation of molecular gradients."
The chemotoxix reacts to the behavior of the organism in response to chemical stimulation. It is well known that the concentration of molecules of lead molecules gradients, such as netrin or semaphorin (Sema) proteins, play a critical role in embryonic nervous development. However, how accurate is the physical profiles of molecular gradients, eg. Neuronal interpretation of the concentration prophylaxis (gradient madness), the interpretation of the neuronal persistence has continued unanswered. Partially reason was the lack of 3-D devices that could reassure important properties of brain tissues outside the human body. Chemotherapy patients often have 2-D, low bandwidth (which means that it must repeat experiments many times to collect data for different parameters)
In response, the CityU team will develop chemical experiments on a new platform. They developed a Hydrographic Microfluid Platform on High-Capacity 3-D Chimatocactic Analysis and Used Neuronal Sensitivity Study of the Molecular Gradient Cycle, Light Mixing on Nerve Regeneration Mechanism, Gradient Profiles in Leading Molecules by Recognizing Soft Variability.
"Our chip reaches just 1 cm2, But houses are hundreds of suspended microscopic hydrogen cylinders, each of which contains a distinct graded picture to make the 3-D growth of the nervous cells in the environment closely in our brain, "says Associate Professor Shim Peggi, Biomedical Engineering Department (BME), who led the research.
"The main advantage of configuration is high throughput, which means that a large collection of molecular gradient profiles can be checked using one chip in order to create enormous data and the time of experiment can be reduced to 48 hours per month," he explains.
Using a new platform and a strict statistical analysis, the team has identified dramatic diversity and difficulty with different manual molecules in the coding regulation of neuronal development. In particular, for Sema3A, the group found that two alarm systems, namely STK11 and GSK3, are indirectly involved in a steep-dependent chemicactic regulation of coordinated neurot replication and neuron migration.
According to these conclusions, the team once again confirmed that the molecule, Sema3A, is only beneficial in the promotion of cortex regeneration if it is presented in the right graded form of the rat brain that is reported in another article Biomaterials Earlier this year.
"In the case of brain trauma, the nervous system is not easily regenerated, so the proper use of molecules of the brain will help to restore the brain, according to this research, our study is designed to develop a novel therapeutic strategy," adds Dr.
How axons change chemical references mechanical strength
Zhen Xu et al, high throughput three-dimensional chemotactic assays reveal the steepness-dependent complexity of neuron sensation molecular gradients, Nature Communications (2018). DOI: 10.1038 / s41467-018-07186-x