Visualizing "eruption" micro-tube growth


Live cells depend on absolutely warm, protein that creates hollow tube polymer, which is called microtubules, which forms the backbone for moving material. Tubulin-based microtubule scaffolding allows cells to move, keep things in place or moves around them. When the cell division occurs, microbobular fibers move to new cells apart from chromosomes. Cell defects die in tuluvin polymerization.


Microtubule fibers are deep rods made with much smaller tubulin subunits that spontaneously assemble at one end of the rod, but exactly how they inside this crowded environment of life cells was a secret. Now researchers UC Davis discovered a mechanism that puts these blocks on the ground, illustrated with new animation.

"He's going to change how people think microtubule polymerization," said Jawdat Al Bassam, Associate Professor of Molecular and Cellular Biology at UC Davis College of Biological Sciences. Paper census is published in the journal on November 13 eLife.

The work describes the videos of TOGs or Tumor Overexpressed Genes, which operate in the Tubeulin Polymerization Management Act. As the name suggests, TOGs are rich in rapidly dividing cancer cells. They show a similar structure for yeast in the body.

The work yeast, the project scientist Stanley Natyante, Al-Bassam and colleagues showed how the protein ALP 14, four TOG domains, accelerates the tubulin polymerization into microtubules by four tubulin units carrying the correct end microtubule and neatly unloads them to the right end.

Alp14 is a well-preserved protein group that is necessary for cell division in cell membrane and cell membrane cells. It consists of a flexible lingerie connector with two TOG1 and two TOG2 domains. Add four tubulin units (two TOG domain names) and creates a circle of Toggs with each other and tubulins out.

When the TOG / tubulin circles reach the end of the micro-tube, TOG1 is bounded by the growing end of its tutu, to destabilize the circle so that it is cut off by four tubulins. The name was selected, because this process is locked by a boat's boat.

"A complete surprise that such a decree is an agreed process," said Al-Bassam.

As the micro-layer layer belongs to the tubulin units, they are pulling the TGG to the subsequent cancellation of TUBULLIN. The process explains how many TOGs will accelerate the tubulin assembly for the first time.

The researchers follow the work of Alp14 mutant proteins that are developed with predicted defects in this process to test this mechanism that implements diagnostic tubulin assembly and living out of the living cells. The researchers plan the further research process, including cryoelectron microscopy, which allows them to treat one protein molecule during their natural state.


Learn further:
The molecular car, not the assembly line, starts the microwaves

Მet Information:
Stanley Natyanatam and other structural bases of Tibulin recruitment and assemblies from the microwave polymers with a tumor osteaxpress gene (TOG) domain, eLife (2018). DOI: 10.7554 / eLife.38922

Journal Reference:
eLife

Provided by:
UC Davis